other:Article Title: SMARCA4 is a haploinsufficient B cell lymphoma tumor suppressor that fine-tunes centrocyte cell fate decisions.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Raw single ATAC sequencing FASTQ files This paper GSE254908 Raw ATAC-seq FASTQ files This paper GSE254908 Raw RNA-sequencing FASTQ files This paper GSE254908 Gene expression microarray data for primary BL GEO GSE132929 Experimental models: Cell lines Human: Raji cells ATCC CCL-86 Human: HEK293T cells ATCC RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock# 000664 Mouse: Cd19cre (B6.129P2(C)- Cd19tm1(cre)Cgn/J) The Jackson Laboratory Stock# 006785 Mouse: AID Cre (B6.129P2-Aicdatm1(cre)Mnz/J) The Jackson Laboratory Stock# 007770 Mouse: CD45.1 (B6.SJL-PtprcaPepcb/Boy) The Jackson Laboratory Stock# 002014 Mouse: Rag1 KO (B6.129S7-Rag1tm1Mom/J) The Jackson Laboratory Stock# 002216 Mouse: R26StopFLMYC (C57BL/6N-Gt (ROSA)26Sortm13(CAG-MYC,-CD2*)Rsky/J) The Jackson Laboratory Stock# 020458 Mouse: GFP-Myc:B6;129-Myctm1Slek/J The Jackson Laboratory Stock#021935 Oligonucleotides gRNA1 for the N-terminal SMARCA4 tagging: TCTGGAGTGGACATCTTCAC This manuscript N/A gRNA2 for the N-terminal SMARCA4 tagging: GTCTGGAGTGGACATCTTCA This manuscript N/A AID-GFP-SMARCA4 N-terminal genotyping Fwd: 5’-CGTCTCCCAAGTTCAAGCGATT-3’ This manuscript N/A AID-GFP-SMARCA4 N-terminal genotyping Rev: 5’-ACATCCAGGGACATGCAAAAGA-3’ This manuscript N/A gRNA1 for knock-in of SMARCA4 point mutation: CAGGACCGAGCCCACCGCAT This manuscript N/A gRNA2 for knock-in of SMARCA4 point mutation: CCGCATCGGGCAGCAGAACG This manuscript N/A Smarca4 mouse genotyping Fwd: 5’—3’ This manuscript N/A Smarca4 mouse genotyping Rev: 5’—3’ This manuscript N/A AID-Cre mouse genotyping common Fwd: 5’- GGACCCAACCCAGGAGGCAGATGT-3’ Robbiani et al.66 N/A AID-Cre mouse genotyping mutant Rev: 5’- CACTCGTTGCATCGACCGGTAATG-3’ Robbiani et al.66 N/A AID-Cre mouse genotyping wildtype Rev: 5’- CCTCTAAGGCTTCGCTGTTATTACCAC-3’ Robbiani et al.66 N/A Myc-KI mouse wildtype Fwd: 5’-CCAAAGTCGCTCTGAGTTGTTATC-3’ Calado et al.25 N/A Myc-KI mouse wildtype Rev: 5’-GAGCGGGAGAAATGGATATG-3’ Calado et al.25 N/A Myc-KI mouse mutant Fwd: 5’-CCAAGAGGGTCAAGTTGGA-3’ Calado et al.25 N/A Myc-KI mouse mutant Rev: 5’-GCAATATGGTGGAAAATAAC-3’ Calado et al.25 N/A GFP-myc mouse common Fwd: 5’- CTGAAAGGGGAGTGGTTCAG-3’ Huang et al.28 N/A GFP-myc mouse wildtype Rev: 5’- ACCGCAACATAGGATGGAGA-3’ Huang et al.28 N/A GFP-myc mouse mutant Rev: 5’- GAACTTCAGGGTCAGCTTGC-3’ Huang et al.28 N/A (Continued on next page) e4 Cancer Cell 42, 605–622.e1–e11, April 8, 2024
Transfection:
Electrophoresis:
Gel Extraction:
Sequencing:
Protein-Protein interactions:
Control:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
Chromatin Immunoprecipitation:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
Recombinant:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Electron Microscopy:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
Polymerase Chain Reaction:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
Purification:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
Staining:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
RNA Sequencing:
Article Title: Antigen-specific T cell responses in SARS-CoV-2 mRNA-vaccinated children.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rabbit IgG An5body, CUTANA CUT&RUN Nega5ve Control EpiCypher 13-0042 An5-Rabbit Secondary An5body for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0047 CUTANA E. coli Spike-in DNA EpiCypher 18-1401 Anti-Mouse Secondary Antibody for CUTANA ChIC/CUT&Tag Workflows EpiCypher 13-0048 CUTANA Concanavalin A Conjugated Paramagnetic Beads EpiCypher 21-1401 Histone H3K27ac Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0045; RRID: AB_2923489 Histone H3K4me1 Antibody, SNAP-ChIP Certified EpiCypher Cat# 13-0040; RRID: AB_2923143 H3K27me3 Monoclonal Antibody (G.299.10), ChIP-Verified ThermoFisher Scientific Cat# MA5-11198; RRID: AB_11000749 Acetyl-Histone H3 (Lys27) Monoclonal Antibody, Unconjugated, Species Reactivity: Human, Host: Mouse / IgG1 ThermoFisherScientific Cat# MA5-23516; RRID: AB_2608307 Chemicals, peptides, and recombinant proteins Deoxyribonuclease I from bovine pancreas Sigma-Aldrich DN25-100MG Collagenase from Clostridium Sigma-Aldrich C9263-500MG Collagenase D Sigma-Aldrich 11088882001 Dispase, neutral protease grade II Roche 4942078001 Trypsin inhibtor from Glycine max (soybean) Sigma-Aldrich T9003-250MG Tamoxifen Sigma-Aldrich T5648-5G Corn oil Sigma-Aldrich C8267-500ML 20% Paraformaldehyde Aqueous Solution, EM Grade Electron Microscopy Sciences 15713-S Histo-Clear II National Diagnostics HS2021GLL EGTA (Ethylene glycol-bis(2-aminoethylether)N,N,N0,N0-tetraacetic acid) Sigma-Aldrich E3889-100G TRIzol LS Reagent Invitrogen 10296028 Recombinant Human TGFalpha R&D Systems 239-A-100 ACK lysing buffer ThermoFisher Scientific A1049201 Caerulein Bachem 4030451.0005 Matrigel Matrix Corning 356231 Dexamethasone Sigma-Aldrich D8893-1MG DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) ThermoFisher Scientific Cat# D3571, RRID:AB_2307445 TDE1 Tagment DNA Enzyme and TD Buffer Illumina 20034198 Trametinib diet (5mg/kg) Bio-Serv S10136 Zombie Aqua Fixable Viability Kit 100 tests BioLegend 423101 Critical commercial assays Agilent High Sensitivity DNA kit Agilent 5067-4626 MinElute PCR Purification Kit Qiagen 28006 Alcian Blue (pH 2.5) Stain Kit Vector Laboratories H-3501 Agilent RNA 6000 Nano Kit Agilent 5067-1511 Deposited data Raw and analyzed data (scRNA-seq; bulk RNA-seq; ATAC-seq) This paper GEO: GSE198564 (Continued on next page) ll Article Developmental Cell 58, 2959–2973.e1–e7, December 18, 2023 e2
Protease Inhibitor:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Microelectrode Array:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Reverse Transcription:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Magnetic Beads:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Single Cell:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Gene Expression:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Cross-linking Immunoprecipitation:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Irradiation:Article Title: Single-nucleus multiomic mapping of m 6 A methylomes and transcriptomes in native populations of cells with sn-m6A-CT.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea Pig Polyclonal anti-Rabbit IgG (H+L) (Secondary Antibody) Novus Biologicals Cat#NBP1-72763; RRID:AB_11024108 Rabbit Polyclonal anti-m6A Synaptic Systems Cat#202003; RRID:AB_2279214 Rabbit Polyclonal anti-m6A Merck Millipore Cat#ABE572-I; RRID:AB_2892214 Chemicals, peptides, and recombinant proteins RiboLock RNase Inhibitor Thermo Scientific Cat#EO0381 cOmplete Protease Inhibitor, EDTA-free Roche Cat#11836170001 pAG-Tn5 (MEA&B loaded) EpiCypher Cat#15-1117 5M Betaine Sigma-Aldrich Cat#B0300-1VL SuperScript IV Reverse Transcriptase Invitrogen Cat#18090010 5X SSIV buffer Invitrogen Cat#18090010 0.1M DTT Invitrogen Cat#18090010 dNTP Mix (25 mM each) Thermo Scientific Cat#R1121 Q5 High-Fidelity 2X Master Mix NEB Cat#M0492S Concanavalin A Conjugated Paramagnetic Beads EpiCypher Cat#21-1411 SPRIselect Magnetic Beads Beckman Coulter Cat#B23318 Critical commercial assays Chromium Next GEM Single Cell Multiome ATAC + Gene Expression 10X Genomics Cat#1000285 Bioanalyzer High Sensitivity DNA Kit Agilent Technologies Cat#50674626 Deposited data Raw and analyzed data This paper GEO: GSE217256 Bulk mESC miCLIP data Ke et al.28 GEO: GSE86336 Bulk mESC MeRIP-Seq data Chelmicki et al.29 GEO: GSE145315 Bulk mESC MeRIP-Seq data Liu et al. 52 GEO: GSE146465 Bulk mESC MeRIP-Seq data Xu et al.33 GEO: GSE154138 Bulk mESC MeRIP-Seq data Liu et al.53 GEO: GSE133599 Bulk mESC MeRIP-Seq data Batista et al.30 GEO: GSE52662 Bulk MEF miCLIP data Boulias et al. 54 GEO: GSE122948 Bulk HEK293T miCLIP data Garcia-Campos et al.7 GEO: GSE122956 Bulk mESC Ythdf1-3 CLIP-Seq data Lasman et al.38 GEO: GSE148039 Single-cell HEK293T DART-seq data Tegowski et al.17 GEO: GSE180954 Experimental models: Cell lines Mouse : E14 embryonic stem cell ATCC CRL-1821 Mouse : CF1 embryonic fibroblast, irradiated MTI-GlobalStem GSC-6001G Mouse : C57BL/6 embryonic stem cell Laboratory of Jacob H. Hanna N/A Mouse : C57BL/6 embryonic stem cell, Mettl3-/- (KO) Laboratory of Jacob H. Hanna N/A Oligonucleotides See Table S4 for the sequences of DNA oligonucleotides used in m6A-CT experiments This paper N/A (Continued on next page) Molecular Cell 83, 3205–3216.e1–e5, September 7, 2023 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Software and algorithms Trim Galore Trim Galore https://github.com/FelixKrueger/ TrimGalore STAR Dobin et al.55 https://github.com/alexdobin/STAR featureCounts Liao et al.56 https://subread.sourceforge.net/ DESeq2 Love et al.57 http://www.bioconductor.org/packages/ release/bioc/html/DESeq2.html deepTools Ramı́rez et al.58 https://github.com/deeptools/deepTools Integrative Genomics Viewer Thorvaldsdóttir et al.59 https://software.broadinstitute.org/ software/igv/ edgeR Robinson et al.60 https://bioconductor.org/packages/ release/bioc/html/edgeR.html Macs2 Macs2 https://pypi.org/project/MACS2/ ChIPseeker Yu et al.61 https://guangchuangyu.github.io/software/ ChIPseeker/ Guitar Cui et al.62 https://bioconductor.org/packages/ release/bioc/html/Guitar.html Homer Heinz et al.64 http://homer.ucsd.edu/homer/ exomePeak2 exomePeak2 https://github.com/ZW-xjtlu/exomePeak2 bedtools Quinlan and Hall.65 https://bedtools.readthedocs.io/en/latest/ GSEA Subramanian et al.66 https://github.com/nungerleider/pyGSEA MeTPeak Cui et al.63 https://github.com/compgenomics/ MeTPeak cellranger Zheng et al.67 https://support.10xgenomics.com/single- cell-gene-expression/software/pipelines/ latest/what-is-cell-ranger Seurat Hao et al.34 https://satijalab.org/seurat/index.html Signac Stuart et al.35 https://stuartlab.org/signac/ SCP SCP https://github.com/zhanghao-njmu/SCP Rmagic van Dijk et al.68 https://github.com/cran/Rmagic Metascape Zhou et al.69 https://metascape.org/ Nebulosa Alquicira-Hernandez and Powell70 https://www.bioconductor.org/packages/ release/bioc/html/Nebulosa.html BioRender BioRender https://biorender.com Other Step by step protocol for m6A-CT This paper See supplemental information
Cell Isolation:
Co-Immunoprecipitation Assay:
Library Quantification:
Microarray:
|